Influence of Growth Regulators on Root and Shoot Initiation from Flax Meristem-Tips and Hypocotyls in vitro |
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| Authors: | W. DAVID LANE |
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| Affiliation: | Agriculture Canada Research Station, Summerland, British Columbia, Canada V0H 1Z0 |
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| Abstract: | Hypocotyl sections and isolated meristem-tips from 7- to 10-day-old seedlings of flax were cultured on a Murashige and Skoog (1962) medium. The optimum concentrations of naphthaleneacetic acid (NAA) and benzyladenine (BA) were determined for shoot and root initiation from hypocotyls. Meristem-tip cultures differentiated shoots and roots in response to these concentrations. The frequency and yield of shoots, promoted by BA, decreased if NAA or gibberellic acid (GA3) was included in the medium. At high NAA concentration GA3 and BA inhibited root initiation. Morphological development of cultures differed depending on the growth regulators in the medium. |
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