Nitric oxide affecting root growth,lignification and related enzymes in soybean seedlings |
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Authors: | Franciele Mara Lucca Zanardo Böhm Maria de Lourdes Lucio Ferrarese Daniele Iara Lucca Zanardo Jose Ronaldo Magalhaes Osvaldo Ferrarese-Filho |
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Institution: | 1.Laboratory of Plant Biochemistry, Department of Biochemistry,University of Maringá,Maringá, PR,Brazil |
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Abstract: | This study analyzed the involvement of nitric oxide (NO) in the root lignification of soybean seedlings. To this end, changes
in root cell viability; phenylalanine ammonia-lyase (PAL) and soluble and cell wall bound peroxidase (POD) activities and
lignin and hydrogen peroxide (H2O2) contents of soybean roots treated with the NO-donor sodium nitroprusside (SNP) and its relationships with root growth were
evaluated. Seedlings were cultivated in a nutrient solution supplemented with 5 to 1,000 μM SNP for 24 h. At an extremely
low concentration (5 μM), SNP induced root growth and increased lignification and activities of related enzymes (PAL and cell
wall-bound POD). At a high concentration (1,000 μM), SNP reduced root growth and lignification (PAL activity and H2O2 and lignin contents) and caused a loss of cell viability. Application of potassium ferrocyanide (an analog of SNP that cannot
release NO) and PTIO (2-phenyl-4,4,5,5,-tetramethylimidazoleline-1-oxyl-3-oxide, a scavenger of NO) revealed that the inhibitory/stimulatory
effects on root lignification may be due to NO itself. These results indicate that NO, depending on its concentration, may
act as a stress factor, due to its toxic action, or as a signal molecule, inducing soybean root growth and lignification. |
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