Pulse treatments of penicillin-G and streptomycin minimise internal infections and have post-treatment effects on the morphogenesis of ginseng root culture |
| |
Authors: | W L Teng L Nicholson |
| |
Institution: | (1) Hong Kong Institute of Biotechnology, 2 Biotechnology Avenue, 12 Miles, Tai Po Road, Shatin, N.T., Hong Kong |
| |
Abstract: | This study was initiated to determine whether antibiotic pulse treatments (APT) could effectively eliminate internal infections of ginseng (Panax ginseng) root explants containing vascular tissue, and subsequently have post-treatment effects on changing explant behaviors in callus induction and organogenesis or somatic embryogenesis. For contamination control, a treatment of 40 min with an antibiotic solution consisting of 1000 mg/1 of penicillin-G and 1000 mg/1 of streptomycin immediately following Na-hypochlorite sterilisation significantly decreased contamination rate. Extending treatment time to 2–3 h further lowered the contamination rate to 30–40%. On the other hand, explants treated with antibiotics for 20 min or less were all contaminated. APT also had post-treatment effects; it delayed callus induction for 1–12 months depending on pulse duration and stimulated the generation of more hardand darker looking than fragile- and lighter looking callus. The induced callus proliferated at a depressed rate, increasing subculture intervals from 1 to several weeks, and not until after five subcultures did it fully recover. The regeneration ability of the recovered callus was also affected by APT: the regeneration of adventitious roots was promoted, whereas somatic embryos were not observed.Abbreviations
APT
Antibiotic pulse treatments
-
2,4-D
2,4-dichlorophenoxyacetic acid |
| |
Keywords: | Panax ginseng Antibiotics Callus Adventitious roots Somatic embryo In vitro |
本文献已被 SpringerLink 等数据库收录! |
|