A rapid DNA extraction method for PCR amplification from wetland soils |
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| Authors: | Li J Li B Zhou Y Xu J Zhao J |
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| Institution: | College of Life Sciences, Inner Mongolia University, Huhhot, China. |
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| Abstract: | Aims: We tested a method of rapid DNA extraction from wetland soil samples for use in the polymerase chain reaction. Methods and Results: The glass bead/calcium chloride/SDS method obtained in the present study was compared with the calcium chloride/SDS/enzymatic extraction method and the UltraClean? Soil DNA Isolation Kit. Rapid DNA extraction could be completed within about two hours without purification steps. Conclusions: This study succeeded in establishing a fast soil DNA extraction protocol that can be applied to various environmental sources that are rich in humic acid content. Significance and Impact of the Study: The method provides a technology with high‐quality DNA extraction from soils for testing the diversity of AOB and AOA. |
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| Keywords: | ammonia‐oxidizing archaea ammonia‐oxidizing bacteria calcium chloride glass bead SDS method |
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