L-ascorbic acid (vitamin C) induces the apoptosis of B16 murine melanoma cells via a caspase-8-independent pathway |
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Authors: | Kang Jae Seung Cho Daeho Kim Young-In Hahm Eunsil Yang Yoolhee Kim Daejin Hur Daeyoung Park Hyunjeong Bang Saic Hwang Young Il Lee Wang Jae |
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Institution: | (1) Department of Anatomy and Tumor Immunity Medical Research Center, Seoul National University College of Medicine, 110-799 Seoul, South Korea;(2) Department of Life Science, Sookmyung Women's University, 140-742 Seoul, South Korea;(3) Department of Anatomy, Inje University College of Medicine, 614-735 Pusan, South Korea;(4) Department of Dermatology, St. Mary's Hospital, The Catholic University of Korea, 420-743 Puchon, South Korea;(5) Department of Plastic Surgery, College of Medicine, Sungkyunkwan University, 110-745 Seoul, South Korea;(6) Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-dong Chongno-gu, 110-799 Seoul, South Korea |
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Abstract: | l-Ascorbic acid (vitamin C) has been reported to play a role in the treatment and prevention of cancer. However, its specific mechanistic pathways remain obscure. This study was carried out to identify the sodium ascorbate–induced apoptotic pathway in B16F10 murine melanoma cells. Sodium ascorbate was found to induce the apoptosis of B16F10 murine melanoma in a time- and dose-dependent manner, and this was prevented by pretreatment with N-acetyl-l-cysteine (NAC), a well-known antioxidant. In fact, sodium ascorbate–treated B16F10 melanoma cells showed increased intracellular reactive oxygen species generation (ROS) levels. These results indicate that sodium ascorbate induced apoptosis in B16F10 murine melanoma cells by acting as a prooxidant. We examined the involvement of caspase-8 using a specific caspase-8 inhibitor (z-IETD-fmk) on the sodium ascorbate–induced apoptotic pathway. Cell death was found not to be inhibited by z-IETD-fmk treatment, indicating that sodium ascorbate–induced apoptosis is not mediated by caspase-8. In addition, we detected a reduction in the mitochondrial membrane potential during apoptosis and confirmed cytochrome-c release from mitochondria by immunoblotting. Taken together, it appears that the induction of a prooxidant state by sodium ascorbate and a subsequent reduction in mitochondrial membrane potential are involved in the apoptotic pathway of B16F10 murine melanoma cells, and that this occurs in a caspase-8–independent manner.Abbreviations NAC N-acetyl-l-cysteine - ROS reactive oxygen species - m mitochondrial membrane potentialJae Seung Kang and Daeho Cho contributed equally to this work. |
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Keywords: | Vitamin C Melanoma Apoptosis |
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