Camptosemin, a tetrameric lectin of Camptosema ellipticum: structural and functional analysis |
| |
Authors: | Fernanda A. H. Batista Leandro S. Goto Wanius Garcia Derminda I. de Moraes Mario de Oliveira Neto Igor Polikarpov Marcia R. Cominetti Heloísa S. Selistre-de-Araújo Leila M. Beltramini Ana Paula Ulian Araújo |
| |
Affiliation: | 1. Centro de Biotecnologia Molecular Estrutural (CBME), Instituto de Física de S?o Carlos (IFSC), Universidade de S?o Paulo (USP), S?o Carlos, Brazil 2. Programa de Pós-gradua??o em Genética e Evolu??o, Universidade Federal de S?o Carlos (UFSCar), S?o Carlos, Brazil 3. Departamento de Ciências Fisiológicas, Universidade Federal de S?o Carlos (UFSCar), S?o Carlos, Brazil 4. Grupo de Biofísica Molecular “Sérgio Mascarenhas”, Instituto de Física de S?o Carlos, Universidade de S?o Paulo, Caixa Postal 369, S?o Carlos, 13560-970, Brazil
|
| |
Abstract: | Lectins have been classified into a structurally diverse group of proteins that bind carbohydrates and glycoconjugates with high specificity. They are extremely useful molecules in the characterization of saccharides, as drug delivery mediators, and even as cellular surface makers. In this study, we present camptosemin, a new lectin from Camptosema ellipticum. It was characterized as an N-acetyl-d-galactosamine-binding homo-tetrameric lectin, with a molecular weight around 26 kDa/monomers. The monomers were stable over a wide range of pH values and exhibited pH-dependent oligomerization. Camptosemin promoted adhesion of breast cancer cells and hemagglutination, and both activities were inhibited by its binding of sugar. The stability and unfolding/folding behavior of this lectin was characterized using fluorescence and far-UV circular dichroism spectroscopies. The results indicate that chemical unfolding of camptosemin proceeds as a two-state monomer-tetramer process. In addition, small-angle X-ray scattering shows that camptosemin behaves as a soluble and stable homo-tetramer molecule in solution. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|