Preliminary characterization of a soluble immunosuppressive molecule from DBA/2 spleen cells using monoclonal antibody immunoadsorbence |
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| Authors: | J K Steele A T Stammers A Chan T Maier J G Levy |
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| Affiliation: | 1. Internal Medicine Division of Allergy and Clinical Immunology, Ege University Medical Faculty, İzmir, Turkey;3. Department of Medical Genetics, Ege University Medical Faculty, İzmir, Turkey;6. Department of Dermatology, Ege University Medical Faculty, İzmir, Turkey;2. Center for Chronic Immunodeficiency, University Medical Center Freiburg and University of Freiburg, Freiburg, Germany;4. Institute of Pathology, University Medical Center Freiburg, Freiburg, Germany;7. Department of Internal Medicine II, University Medical Center Freiburg, Freiburg, Germany;5. Grigore T. Popa University of Medicine and Pharmacy, Department of Immunology, Iasi, Romania;8. Department of Pediatric Immunology and Allergy, Ankara University School of Medicine, Ankara, Turkey;9. Institute for Transfusion Medicine, University of Ulm, and the Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Service, Baden-Württemberg-Hessen, Ulm, Germany;1. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Hohe Strasse 6, 01069 Dresden, Germany;2. Chair of Food Chemistry and Molecular Sensory Science, Technical University of Munich, Lise-Meitner-Straße 34, 85354 Freising, Germany;3. Leibniz-Institute for Food Systems Biology at the Technical University of Munich, Lise-Meitner-Straße 34, 85354 Freising, Germany;4. Clinic of Operative and Pediatric Dentistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, D-01307 Dresden, Germany;5. Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, Building 73, 66421 Homburg/Saar, Germany;6. Technische Universität Dresden, Center for Regenerative Therapies Dresden, Tatzberg 47, 01307 Dresden, Germany;1. Division of Allergy & Clinical Immunology, University of Virginia, Charlottesville, Va;2. Allergy Partners of Lynchburg, Lynchburg, Va;3. Essentia Health, Duluth, Minn;4. Allergy Clinic of Tulsa, Tulsa, Okla;5. Department of Public Health and Clinical Medicine, OLIN Unit, Umeå University, Umeå, Sweden;6. Pediatric and Adult Asthma and Allergy, Birmingham, Ala;7. Division of Rheumatology, Allergy & Immunology, University of North Carolina, Chapel Hill, NC;8. The Allergy and Asthma Clinic of Northwest Arkansas, Bentonville, Ark |
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| Abstract: | In a previous publication a monoclonal antibody (B16G) which appeared to recognize T suppressor cells and a T-suppressor factor (TsF) in the spleens of DBA/2 mice was described. B16G appears to be directed to a public specificity of DBA/2 TsF and therefore has been shown to inhibit a variety of immunological reactions. The present study involves preliminary characterization of the material with which B16G reacts. It was found that the B16G-reactive protein (putative TsF) could be absorbed and eluted specifically from a B16G immunoadsorbent column. Material eluting from the B16G column reacted with B16G in an ELISA and appeared to run as two or more bands of 40-45 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The eluted material was biologically active (i.e., suppressive) in the standard assay (mixed leukocyte reaction of DBA/2 splenocytes with B10.BR targets), and its suppressive activity was abrogated by the addition of B16G to the mixed leukocyte reaction cultures. Sephadex G-150 chromatography of the B16G-reactive material showed that under these conditions, its native molecular mass was between 80-90 kDa, indicating that it might occur as a dimer under natural conditions. |
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