Bacterial DNA decontamination for reverse transcription polymerase chain reaction (RT-PCR) |
| |
| Authors: | Wang Gehua Barton Connie Rodgers Frank G |
| |
| Affiliation: | National Laboratory for Enteric Pathogens, National Microbiology Laboratory, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2. |
| |
| Abstract: | For RT-PCR, removal of contaminating genomic DNA in RNA samples using manganese sulfate was more effective than magnesium. DNA contamination was removed in 3 microg of nucleic acid using 10 U of RNase-free DNase I in 10-microl reaction volumes. The digestion procedure was compatible with commercial RNA extraction kits and was suitable for RT-PCR assay. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
