Unscheduled DNA synthesis correlated to alkylation of hemoglobin in individuals occupationally exposed to propylene oxide |
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| Authors: | Ronald W. Pero Siv Osterman-Golkar Benkt Högstedt |
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| Affiliation: | (1) Wallenberg Laboratory, Molecular Ecogenetics, University of Lund, Lund, Sweden;(2) Department of Radiobiology, Stockholm University, Stockholm, Sweden;(3) Department of Occupational Medicine, District Hospital, Halmstad, Sweden;(4) Wallenberg Laboratory, Molecular Ecogenetics, University of Lund, Box 7031, S-220 07 Lund, Sweden |
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| Abstract: | Exposure to propylene oxide was determined previously by the degree of alkylation of hemoglobin measured on the histidine residue as N-3-(2-hydroxypropyl) histidine, using blood samples from 8 propylene oxide-exposed employees and 13 unexposed referents. Mononuclear leukocytes isolated from the same blood samples were used to quantify DNA repair proficiency following an in vitro challenge with the carcinogen, N-acetoxy-2-acetylamino-fluorene. Decreases in the DNA repair proficiency index correlated significantly to in vivo exposure levels to propylene oxide (r = –0.64, p <0.03). These data suggest a possible short-term biological assay for monitoring the in vivo genotoxic effects of propylene oxide exposure in the human population.Abbreviations EO ethylene oxide - NA-AAF N-acetoxy-2-acetylaminofluorene - HOPrHIS N-3-(2-hydroxypropyl) histidine - PO propylene oxide - UDS unscheduled DNA synthesis |
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| Keywords: | genotoxicity hemoglobin alkylation propylene oxide unscheduled DNA synthesis |
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