河南花背蟾蜍的核型、C-带和Ag-NORs研究

利用骨髓细胞蒸汽固定法制备染色体标本，研究了分布于河南新乡的花背蟾蜍(Bufo raddei)的核型、C-带和Ag-NORs。结果表明：河南产花背蟾蜍体细胞染色体数为22条，核型公式为2n=22(20m 2sm)，全部为中部或亚中部着丝点染色体，NF=44。Ag-NORs具有多态现象。C-带核型显示22条染色体的着丝点均正染，No．1染色体近着丝粒处有不恒定插入型C-带，No．4染色体具有恒定近端部插入型C-带，随体部位被正染的仅占所观察细胞的15％。

Karyotype,C-banding and Ag-NORs of Bufo raddei from Henan

The chromosome samples were prepared from metaphase marrow cells from Bufo rad dei which distributed in Xinxiang, Henan by steam-fixation method. Karyotype, C-banding and silver-staining nucleolus organizer regions (Ag-NORs) were obse rved on chromosomes. The chromosome number of Bufo raddei is 2n=22(20m 2sm), and all of the chromosomes were metacentric or submetacentric (NF=44). In additi on, polymorphism was found in Ag-NORs whereas all the chromosomes were stained in C-banding. There was a variable insert type C-banding near centromere in No .1 chromosome and an invariable insert type C-banding near terminal position in No.4 chromosome.