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Extracellular chitinases of mutant superproducing strain Serratia marcescens M-1
Authors:A. B. Duzhak  Z. I. Panfilova  T. G. Duzhak  E. A. Vasyunina
Affiliation:(1) Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, pr. Akademika Lavrentieva 10, 630090 Novosibirsk, Russia;(2) University of Kansas, 2121 Simons Drive, Lawrence, KS 66047, USA
Abstract:Four extracellular proteins with chitinase activity capable of binding chitin substrates have been revealed in the culture liquid of chitinase superproducing mutant strain M-1 of Serratia marcescens. Proteins were analyzed by SDS-PAGE and MALDI-TOF mass spectrometry. Based on the data obtained, the proteins were identified as typical chitinases of S. marcescens: ChiA, ChiB, ChiC, and CBP21.
Keywords:chitinase  colloidal chitin   Serratia marcescens   mass spectrometry
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