<Emphasis Type="Italic">FLEXIBLE CULM 1</Emphasis> encoding a cinnamyl-alcohol dehydrogenase controls culm mechanical strength in rice |
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Authors: | Xiangjun Li Ying Yang Jialing Yao Guoxing Chen Xianghua Li Qifa Zhang Changyin Wu |
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Institution: | (1) National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China |
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Abstract: | Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control
culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including
late heading time, semi-dwarf habit, and flexible culm. In this study, we cloned the FLEXIBLE CULM1 (FC1) gene in rice using a T-DNA tagging approach. FC1 encodes a cinnamyl-alcohol dehydrogenase and is mainly expressed in the sclerenchyma cells of the secondary cell wall and
vascular bundle region. In these types of cells, a deficiency of FC1 in the fc1 mutant caused a reduction in cell wall thickness, as well as a decrease in lignin. Extracts from the first internodes and
panicles of the fc1 plants exhibited drastically reduced cinnamyl-alcohol dehydrogenase activity. Further histological and biochemical analyses
revealed that the p-hydroxyphenyl and guaiacyl monomers in fc1 cell wall were reduced greatly. Our results indicated that FC1 plays an important role in the biosynthesis of lignin and the control of culm strength in rice.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | FLEXIBLE CULM 1 Lignin biosynthesis Cinnamyl-alcohol dehydrogenase Rice |
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