| 应用反义RNA技术降低肿瘤细胞的耐药性 |
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| 引用本文： | 季守平,吴英,侯春梅,由英,毛宁,章扬培.应用反义RNA技术降低肿瘤细胞的耐药性[J].中国生物化学与分子生物学报,1999,15(1):157-158. |
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| 作者姓名： | 季守平 吴英 侯春梅 由英 毛宁 章扬培 |
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| 作者单位： | 军事医学科学院基础医学研究所 |
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| 摘 要： | 细胞内的Ｏ６－甲基鸟嘌呤－ＤＮＡ甲基转移酶（ＭＧＭＴ）能够修复亚硝脲药物造成的ＤＮＡ损伤，阻止亚硝脲药物对肿瘤的杀伤作用，使细胞对亚硝脲药物产生耐药性．我们曾经构建了三个表达ＭＧＭＴ反义ＲＮＡ的逆转录病毒载体，导入对亚硝脲呈抗性的ＨｅＬａＳ３细胞，并...
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| 收稿时间： | 1999-02-20 |
Decrease of Tumor Drug Resistance by Antisense RNA Technique |
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| JI Shouping,WU Ying,HOU Chunmei,YOU Ying,MAO Ning,ZHANG Yangpei.Decrease of Tumor Drug Resistance by Antisense RNA Technique[J].Chinese Journal of Biochemistry and Molecular Biology,1999,15(1):157-158. |
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| Authors: | JI Shouping WU Ying HOU Chunmei YOU Ying MAO Ning ZHANG Yangpei |
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| Institution: | (Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850 |
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| Abstract: | Decrease of tumor cellular resistance to ACNU by retroviral mediated antisense RNA targeting 5′ region of O 6 methylguanine DNA methyltransferase(MGMT)mRNA was reported.It was found that after MGMT gene expression was depleted by antisense RNA,the tumor implanted in nude mice exhibited to be more sensitive to the killing effect of ACNU.It suggested that appropriate antisense RNA might deplete MGMT activity in human tumor cells and decrease drug resistance to ACNU. |
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| Keywords: | Antisense RNA Drug resistance ACNU |
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