Modified binary hammerhead ribozymes with high catalytic activity |
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Authors: | Vorobjeva M Gusseva E Repkova M Kovalev N Zenkova M Venyaminova A Vlassov V |
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Institution: | Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia. |
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Abstract: | A series of binary hammerhead ribozymes was designed and assessed in terms of cleavage activity and nuclease resistance. Enhanced nuclease resistance of binary ribozymes was achieved by incorporation of Z-modified nucleotides at the selective positions along with addition of 3'-3-linked thymidine cap. These modified binary ribozymes efficiently cleave 190-nucleotides long MDR1 mRNA fragment and display catalytic activity much higher then respective full-length analogs. |
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