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The effect of light quality on anther culture of Citrus clementina Hort. ex Tan.
Authors:Germanà Maria Antonietta  Chiancone Benedetta  Iacona Calogero  Muleo Rosario
Affiliation:(1) Dipartimento S.EN.FI.MI.ZO. Sezione Frutticoltura Mediterranea, Tropicale e Subtropicale. Facoltà di Agraria, Università degli Studi di Palermo, Viale delle Scienze, 11, 90128 Palermo, Italy;(2) Dipartimento di C. D. S. L., Università degli Studi di Pisa, Via del Borghetto 80, 56124 Pisa, Italy;(3) Dipartimento di Pro.V. Facoltà di Agraria, Università degli Studi della Tuscia, Via San Camillo de Lellis s.n.c., 01100 Viterbo, Italy
Abstract:This preliminary research reports results on the influence of light quality on anther culture of Citrus clementina Hort. ex Tan., cultivar Nules. After one month of cultivation in darkness, four light qualities were tested: White, Red, Far-Red and Blue. Continuous Darkness and White light under photoperiod of 16 hrs were used as a control conditions. Gametic embryoids and embryogenic callus were obtained only under photoperiodic conditions of White light, suggesting that the alternation of light and dark can be used for the process of gametic embryogenesis in Citrus.
Keywords:anther culture   Citrus   haploids  light quality
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