Metabolite profiling of potato (Solanum tuberosum L.) tubers during wound-induced suberization |
| |
Authors: | Wei-Li Yang Mark A Bernards |
| |
Institution: | (1) Environmental Stress Biology Group, Department of Biology, The University of Western Ontario, North Campus Building, London, ON, N6A 5B7, Canada |
| |
Abstract: | Suberin is a specific cell wall-associated biopolymer characterized by the deposition of both a poly(phenolic) domain (SPPD)
associated with the cell wall, and a poly(aliphatic) domain (SPAD) thought to be deposited between the cell wall and plasma
membrane. In planta, suberin functions to prevent plants from desiccation and pathogen attack. Although the chemical identity of the monomeric
components of the SPPD and SPAD are well known, their concerted biosynthesis and assembly into the suberin macromolecule is
poorly understood. To expand our knowledge of suberin biosynthesis, a GC/MS-based metabolite profiling study was conducted,
using wound healing potato (Solanum tuberosum L.) tubers as a model system. A time series of both non-polar and polar metabolite profiles were created, yielding a broad-based,
dynamic picture of wound-induced metabolism, including suberization. Principal component analysis revealed a separation of
metabolite profiles according to different suberization stages, with clear temporal differences emerging in the non-polar
and polar profiles. In the non-polar profiles, suberin-associated aliphatics contributed the most to cluster formation, while
a broader range of metabolites (including organic acids, sugars, amino acids and phenylpropanoids) influenced cluster formation
amongst polar profiles. Pair-wise correlation analysis revealed strong correlations between known suberin-associated compounds,
as well as between suberin-associated compounds and several un-identified metabolites in the profiles. These data may help
to identify additional, as yet unknown metabolites associated with suberization process. |
| |
Keywords: | suberin potato metabolite profiling PCA correlation analysis |
本文献已被 SpringerLink 等数据库收录! |
|