Probing DNA triple helix structure by chemical ligation |
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| Authors: | N G Dolinnaya O V Pyatrauskene Z A Shabarova |
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| Institution: | A.N. Belozersky Laboratory and Chemical Department, Moscow State University, USSR. |
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| Abstract: | A series of oligomeric double and triple helical DNAs with irregular sequences of homopurine and homopyrimidine strands were prepared. DNA triplexes were identified by CD spectroscopy and thermal denaturation profiles (biphasic helix-coil transition). Condensation of oligonucleotides on single and double-stranded DNA templates was performed using water-soluble carbodiimide, phosphodiester and pyrophosphate internucleotide bonds being newly formed. Such chemical ligation proved to be a sensitive monitor of changes in the sugar-phosphate backbone resulting from conversion of double to triple helix and of third-strand binding. |
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