|
|||||
|
|
Production of recombinant rotavirus VP6 from a suspension culture of transgenic tomato (Lycopersicon esculentum Mill.) cells |
|
| Authors: | In Sik Chung Chang Heon Kim Kyung Il Kim Seong Hyun Hong Jong Hwa Park Ju Kon Kim Won Yong Kim |
| Affiliation: | (1) Department of Genetic Engineering, Kyung Hee University, Suwon, 449-701, Korea;(2) Department of Biological Science and Biotechnology, Myung Ji University, Yongin, 449-728, Korea;(3) Department of Medical Microbiology, Chung Ang University, Seoul, 156-756, Korea |
| Abstract: | Recombinant rotavirus VP6 expressed in transgenic tomato cells was found primarily in the intracellular fraction and had a molecular weight of 44 kDa. In a shake flask, transgenic tomato cells produced 0.33 mg recombinant VP6 l–1 after 18 days of incubation. In a high aspect rotating-wall vessel designed by NASA to simulate microgravity, the transgenic cells produced up to 0.15 mg recombinant VP6 l–1. |
| Keywords: | high aspect rotating-wall vessel production rotavirus VP6 transgenic tomato cells |
| 本文献已被 SpringerLink 等数据库收录！ | |