Molecular cloning vectors derived from the CoLE1 type plasmid pMB1.

$\text{In}$$\text{vitro}$ recombination via restriction endonucleases and $\text{in}$$\text{vivo}$ genetic translocation of an impicillin resistance gene, have been utilized for the construction of a series of cloning vehicles. These vectors have been derived by combining the essential replication properties of plasmid pMBl, a CoLEl type plasmid, with one, two or three resistance markers (tetracycline, ampicillin, chloramphenicol and colicin El production). During the construction of these vectors, the position of restriction sites used for cloning DNA fragments, relative to the antibiotic resistance genes and the origin of DNA replication, have been determined. The use of the cloning vectors pMB9, pBR313, pBR322, pBR324, pBR325 permits the molecular cloning and easy selection of $\text{Eco}$ RI, $\text{Bam}$ HI, $\text{Bgl}$ II, $\text{Dpn}$ II, $\text{Hind}$ III, $\text{Sal}$ I, $\text{Xho}$ I, $\text{Xam}$ I, $\text{Taq}$ I, $\text{Pst}$ I, $\text{Hinc}$ II, $\text{Pvu}$ I, $\text{Sma}$ I, $\text{Xma}$ I, $\text{Ba}$l I, $\text{Hpa}$ I, $\text{Ava}$ I, $\text{Pvu}$ II, $\text{Hae}$ III, $\text{Alu}$ I, $\text{Hpa}$ II, $\text{Eco}$ RII and virtually any blunt-ended generated DNA fragment.