The influence of culture medium composition on drug metabolising enzyme activities of the human liver derived Hep G2 cell line |
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| Authors: | H Doostdar S J Duthie M D Burke W T Melvin M H Grant |
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| Institution: | Department of Medicine & Therapeutics, University of Aberdeen, Scotland. |
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| Abstract: | When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10-20% of the cytochrome P-450-dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP-glucuronyltransferase and glutathione-S-transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O-dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50-, 30- and 10-fold, respectively. |
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