Analysis of total proteins in pollen of Humulus scandens Lour in Wuhan Region of China by two-dimensional electrophoresis |
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| Authors: | Dongdong Li Shaoheng He |
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| Institution: | 1. Department of Bioengineering, Hainan University, Haikou, 570228, China
2. Institute of Allergy and Inflammation, Medical Collage of Shantou University, Shantou, 515031, China
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| Abstract: | Total proteins in the pollen of Humulus scandens Lour，one of the most popular aeroallergens in China，were analyzed by two-dimensional electrophoresis in the current study.The proteins were extracted by Trichloracetic acid (TCA) method，and then separated by isoelectric focusing as the first dimension and SDS-PAGE as the second dimension.The spots of proteins were visualized by staining with Coomassie Brilliant Blue.After analysis with software (ImageMaster 2D)，122 different proteins were detected;isoelectric point (pI)，Molecular weight (MW) and relativevolume of each protein in the pollen were also discovered.This is the first high-resolution，two-dimensional protein map of the pollen ofHumulus scandens Lour in China.Our finding has built a solid foundation for identification，characterization，gene cloning and standardization of allergenic proteins in the pollen ofHumulus scandens Lour for further studies. |
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| Keywords: | Humulus scandens Lour pollen allergen proteins two-dimensional electrophoresis |
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