Biochemical characteristics and genetic diversity of <Emphasis Type="Italic">Vibrio</Emphasis> spp. and <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis> strains isolated from the Lac of Bizerte (Tunisia) |
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| Authors: | Snoussi Mejdi Noumi Emira Messaoud Ali Hajlaoui Hafedh Bakhrouf Amina |
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| Institution: | 1.Laboratoire de Traitement des Eaux Usées. Centre de Recherches et des Technologies des eaux,Technop?le de Borj-Cédria,Hammam-Lif,Tunisie;2.Laboratoire d’Analyse, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Département de Microbiologie,Faculté de Pharmacie,Monastir,Tunisie |
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| Abstract: | This study characterized the phenotypic and genetic properties of Vibrio spp. and Aeromonas
hydrophila strains isolated from seawater and mussels (Mytilus
edulis and Crassostrea
gigas) cultured in mollusc farm localized in the lac of Bizerte. The 37 strains (31 strains of V.
alginolyticus, one strain of V. fluvialis, one strain of V. parahaemolyticus and four strains of A.
hydrophila) typed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) showed a high polymorphism. Most of the isolates
were resistant to at least two antimicrobial agents. All the tested strains were resistant to ampicillin. PCR was used to
detect the presence of eight Vibrio
cholerae virulence genes in the genome of the Vibrio spp. isolates. The results showed a wide dissemination of these genes in the genome of all Vibrio spp. isolates tested. Differentiation of these strains with the ERIC 2-PCR technique revealed no association between the
presence of virulence genes and a particular fingerprinting pattern. |
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