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Transfer of IncP plasmids into Stigmatella aurantiaca leading to insertional mutants affected in spore development |
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| Authors: | Ingrid Glomp Patrick Saulnier Janine Guespin-Michel Hans Ulrich Schairer |
| Affiliation: | (1) Zentrum für Molekulare Biologie, Universität Heidelberg, Im Neuenheimer Feld 282, D-6900 Heidelberg 1, Federal Republic of Germany;(2) Laboratoire de Génétique Microbienne, Université de Technologie de Compiègne, B.P. 233, F-60206 Compiègne Cedex, France;(3) Faculté des Sciences, Université de Rouen, B.P. 118, F-76134 Mont-Saint-Aignan Cedex, France |
| Abstract: | Summary Derivatives of the broad-host-range plasmid RP4, containing the wild-type or modified transposon Tn5 were transferred by conjugation to various Stigmatella aurantiaca isolates. The transposons and in some cases fragments of the plasmid as well were integrated into the chromosome. Thus, insertional mutants have been obtained affected in spore formation in liquid culture. |
| Keywords: | Stigmatella aurantiaca IncP Plasmids Insertional mutants Spore development Conjugational transfer |
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