Production of biosurfactants by Cladosporium resinae |
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Authors: | Joaquín M. Muriel José M. Bruque José Manuel Olías Alfonso Jiménez-Sánchez |
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Affiliation: | (1) Departamento de Bioquímica y Biología Molecular y Genética, Universidad de Extremadura, E06080 Badajoz, Spain;(2) Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Extremadura, Badajoz, Spain;(3) Instituto de la Grasa, C.S.I.C., Sevilla, Spain |
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Abstract: | Summary Cladosporium resinae produces extracellular biosurfactants when growing in a hydrocarbon source such as the jet fuel JP8. This production of biosurfactants was observed by the reduction of the surface tension of the aqueous phase of growing medium, and by the increase in emulsion and foaming properties. A partial purification by collapsed foam gave better physical properties by decreasing surface tension and increasing foaming power and stabilization of emulsions. Surface active substances were purified by reversed phase chromatography. Six compounds representing over 75% of fraction containing surface activity were present. This fraction gave an improvement of all surface properties. |
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