Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically |
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Authors: | Ringrose Jeffrey H van Solinge Wouter W Mohammed Shabaz O'Flaherty Martina C van Wijk Richard Heck Albert J R Slijper Monique |
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Affiliation: | Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands. |
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Abstract: | In-depth human erythrocyte proteome studies are severely hampered by the presence of hemoglobin and carbonic anhydrase-1, which account for more than 98% of the total erythrocyte soluble protein content. We developed a specific depletion approach that resulted in a drastic increase in the number of identified proteins. This depletion technique is valuable for proteome studies of human erythrocyte disorders with unknown etiology and of tissue samples that contain blood. |
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