Interactions between M protein and other structural proteins of severe,acute respiratory syndrome-associated coronavirus |
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Authors: | Yi-Ching Hsieh Hui-Chun Li Shih-Chi Chen Shih-Yen Lo |
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Affiliation: | (1) Graduate Institute of Molecular and Cellular Biology, Tzu Chi University, 701, Section 3, Chung Yang Road, Hualien, Taiwan;(2) Graduate Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan;(3) Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan |
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Abstract: | Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) structural proteins (S, E, M, and NC) localize in different subcellular positions when expressed individually. However, SARS-CoV M protein is co-localized almost entirely with S, E, or NC protein when co-expressed in the cells. On the other hand, only partial co-localization was observed when S and E, S and NC, or E and NC were co-expressed in the cells. Interactions between SARS-CoV M and other structural proteins but not interactions between S and E, S and NC, or E and NC were further demonstrated by co-immunoprecipitation assay. These results indicate that SARS-CoV M protein, similar to the M proteins of other coronaviruses, plays a pivotal role in virus assembly. The cytoplasmic C-terminus domain of SARS-CoV M protein was responsible for binding to NC protein. Multiple regions of M protein interacted with E and S proteins. A model for the interactions between SARS-CoV M protein and other structural proteins is proposed. This study helps us better understand protein-protein interactions during viral assembly of SARS-CoV. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | SARS-CoV Membrane protein Structural proteins Co-localization Co-immunoprecipitation |
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