An adaptable spectroelectrochemical titrator: the midpoint reduction potential of the iron-sulfur center in lysine 2,3-aminomutase |
| |
Authors: | Hinckley Glen T Frey Perry A |
| |
Affiliation: | Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 35726, USA. |
| |
Abstract: | Elaborations to an earlier design of an electron paramagnetic resonance (EPR) spectroelectrochemical titrator are described. While maintaining the anaerobic capabilities of the original design, a number of modifications and revisions have been introduced. The most significant modification is the use of a detachable spectral cell, making the apparatus modular and adaptable for multiple forms of spectroscopy. Additional modifications include removable reference, auxiliary, and working electrodes; modifications to facilitate sample transfer; and adaptations for operation within an anaerobic chamber. This apparatus has been used successfully in the coulometric titration of a [4Fe-4S] enzyme, as measured by EPR spectroscopy. The midpoint reduction potential for the 2+/1+ couple in the [4Fe-4S] cluster of lysine 2,3-aminomutase is -479+/-5mV, a value that falls within the range typical of ferredoxin-like iron-sulfur clusters. |
| |
Keywords: | Spectroelectrochemical titrator Lysine 2,3-aminomutase Reduction potential of lysine 2,3-aminomutase Reduction potentials by optical or EPR spectroscopy |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|