后丽盲蝽属作为属级分类单元的DNA分子证据

用蛋白酶K消化法提取并正反链双向测得半翅目盲蝽科盲蝽族(Mirini)中后丽盲蝽属(Apolygus)和草盲蝽属(Lygus)9个种的线粒体DNA细胞色素b基因片段的序列，应用Mega计算该两个属7个种11个Cyt b 432 bp片段序列的碱基组成、核苷酸的种间变异率、所编码的144个氨基酸的变异率。其核苷酸变异率：后丽盲蝽属内小于0．02，草盲蝽属内小于0．08(个别种例外)；两个属种类之间平均变异率都大于0．10，平均为0．15。氨基酸变异率：后丽盲蝽属内种间无变异，而草盲蝽属内只有L．gemellatus与其他种间有13个氨基酸变异，仅占9．03％；两属各种类间却存在8～11个氨基酸的变异。表明在分子水平上两属之间较之于属内种间具有明显的差异，支持将后丽盲蝽从亚属提升为属级分类单元的分类观点。

A DNA Molecular Evidence for Apolygus China as a Generic Taxon (Hemiptera:Heteroptera:Miridae)

Sequences of the Cyt b gene segments on mitochondrial DNA of 9 species of Apolygus China and Lygus Hahn (Heteroptera:Miridae:Mirinae:Mirini) were extracted by means of protease K digesting and sequenced to both directions.Nucleotide composition,nucleotide variation ratio between species as well as variation ratio of 144 amino acid coded by 11 segments of 433?bp of the 9 species from the two genera were calculated using phylogenetic analyzing software package Mega.Nucleotide variation ratio:less than 0.02 within Apolygus and less than 0.08 within Lygus (with the exception of a few species) but more than 0.10 between species of the two groups with the average variation of 0.15.Variation ratio of the amino acid:no difference among the species within Apolygus and Lygus themselves with only an exception of a difference of 13 amino acid between L.gemellatus and other species within Lygus,but there is stably a variation of 8 to 11 amino acids,i.e.a 5.6% to 7.6% variation between any species examined of Apolygus and that of Lygus.It is proved that there has been existed distinct difference between these two groups at molecular level and they separated very well,which strongly supports the taxonomic opinion that Apolygus is promoted to a generic taxon.