Identification of vaccinia promoters by heterologous expression of hepatitis B surface antigen in mouse cells infected by recombinant vaccinia viruses |
| |
Authors: | Tristan Barbeyron, Mohamed Mars, Elisabeth Schroeder, Yves Malpi ce, Andrzej Plucienniczak, Georges Beaud,Rolf E. Streeck |
| |
Affiliation: | Tristan Barbeyron, Mohamed Mars, Elisabeth Schroeder, Yves Malpièce, Andrzej Plucienniczak, Georges Beaud,Rolf E. Streeck, |
| |
Abstract: | DNA fragments preceding open reading frames in a conserved segment of the vaccinia virus genome (Plucienniczak A., et al. (1985) Nucleic Acids Res. 13, 985–998) were cloned into plasmids upstream of the S gene of the hepatitis B virus encoding the surface antigen (HBsAg). Recombinant vaccinia virus obtained after insertion of these constructs into the thymidine kinase gene were used to infect mouse 1D cells. HBsAg was assayed in cellular supernatants. A strong promoter was thus identified in a 295 bp fragment preceding the coding region of the 147 kDa subunit of the vaccinia RNA polymerase. |
| |
Keywords: | Gene expression Virus promoter Hepatitis B surface antigen Recombinant vaccinia virus (Mouse cell) |
本文献已被 ScienceDirect 等数据库收录! |
|