Sequence similarity between Photosystems I and II. Identification of a Photosystem I reaction center transmembrane helix that is similar to transmembrane helix IV of the D2 subunit of Photosystem II and the M subunit of the non-sulfur purple and flexible green bacteria

There are basic structural similarities between plant PS II and bacterial RCs of the Chloroflexaceae and Rhodospirillaceae. These RCs are referred to as PS II-type RCs. A similar relationship of PS I RC to PS II-type RCs has not been established. Although plant PS I and PS II RCs show structural and functional differences, they also share similarities. Therefore, the A and B polypeptides of PS I were searched for PS II D1 and D2 polypeptide-like sequences. An alignment without gaps was found between PS II-type D2/M helix IV and PS I B helix X, as well as a weaker alignment of PS II-type D1/L with PS I B helix X. No comparable alignment with PS I A was found. In the M/D2 alignment there were eight identities and some conservative substitutions in twenty nine residues. PS I B helix X appeared to contain a modified chlorophyll dimer and monomer binding site and a modified non-heme iron-quinone binding site. The conserved residue sequence was found only in RC polypeptides. The proposed chlorophyll dimer-monomer binding site was located transmembrane from the iron-sulfur cluster X binding site. The conserved residues generally are those that interact with prosthetic groups. Half of the conserved residues are located on the same side of the helix. Thus, although there are impediments to concluding firmly that PS I B helix X has a functional and evolutionary relatedness to the D2 PS II and bacterial M RC polypeptides, our analysis gives reasonable support to the idea.Abbreviation RC reaction center