Hydroxylation of oleanolic acid to queretaroic acid by cytochrome P450 from Nonomuraea recticatena |
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Authors: | Fujii Yoshikazu Hirosue Shinji Fujii Tadashi Matsumoto Naoki Agematu Hitosi Arisawa Akira |
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Institution: | Bioresource Laboratories, Mercian Corp., Iwata-shi, Shizuoka, Japan. fujii-ysk@mercian.co.jp |
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Abstract: | A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%. |
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