Studies of the blood chemistry of Electrophorus electricus and a new physiological saline solution based thereon |
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Authors: | George D Webb Burt B Hamrell Donald A Farquharson William D Niemi |
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Institution: | Department of Physiology and Biophysics, University of Vermont College of Medicine, Burlington, Vt. 05401 U.S.A. |
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Abstract: | Based upon analyses of the composition of electric eel blood serum we suggest a new physiological saline solution as follows: 188 mM NaCl, 5 mM KCl, 2 mM MgCl2, 2 mM CaCl2, 0.15 mM NaH2PO4, 1.45 mM Na2HPO4 and 5 mM glucose; pH 7.4. The major difference between this saline and that used in most of the previous investigations using eel electroplaques is that the total Na+ concentration is increased from between 162.7 and 171.7 mequiv/l to 191 mequiv./l. This increase does not appear to affect the electrophysiological properties of the electroplaque. |
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