参杞制剂对小鼠抗疲劳和抗氧化作用研究

目的：探讨参杞制剂抗疲劳及抗氧化作用效果，为抗疲劳制剂的研制提供实验依据。方法：将120只雄性昆明种小鼠按体质量随机平均分为4组，分别为参杞制剂高、中、低剂量组和对照组；分批实验，每次实验从对应组中随机抽取10只小鼠。灌喂实验制剂15d后，分别测定负重力竭游泳时间，血乳酸、血清尿素氮、肝糖原、肌糖原含量及肝脏超氧化物歧化酶（soD）、丙二醛（彻A）、谷胱甘肽过氧化物酶（GsH_h）。结果：参杞制剂低、中、高3个剂量组小鼠的力竭游泳时间（296．0±25．3）s，（437.0±38．9）s，（595．04±53．9）s]均大于对照组（231．04±22．5）s]，其中，高、中剂量组与对照组相比差异显著。运动后，参杞制剂高、中剂量组小鼠的血乳酸水平显著低于对照组；3个剂量组小鼠血尿素氮含量均低于对照组，其中，高、中剂量组与对照组的差异具有显著性；高、中剂量组小鼠肝糖原、肝脏SOD和GSH-Px含量均显著高于对照组；高剂量组小鼠MDA含量明显低于对照组。结论：参杞制剂，尤其是中高剂量组，能明显延长小鼠力竭游泳时间，并具有较强的抗疲劳与抗氧化作用。

Effects of Shenqi preparation on anti-fatigue and anti-oxidant functions in mice

Objective： To explore effects of Shenqi preparation, Traditional Chinese Medicine, on anti-fatigue and anti-oxidant functions. Methods： One hundred and twenty mice were randomly divided into control group and 3 experimental ~oups. The high,medium and low-dose of Shenqi preparation were given to the 3 experimental groups respectively, while distilled water to the control group for 15 d. The loaded swim ming time, the level of lactate, serum urea nitrogen（SUN）, muscle and liver glycogen, liver super-oxide dismutase（SOD）, the content of malon dialdehyde （MI）A）, glutathione peroxidase（GSH-Px） were assayed. Results： The loaded swimming test showed that the exhausted swimming time of 3 experimental groups （296,0 ± 25.3 ） s, （437.0± 38.9） s, （595.0 ± 53.9） s respectively ] was longer than that of control group （231.0 ± 22.5）s, P 〈 0.05, P 〈 0.01 ]. The liver glycogen content of the high and medium-dose experimental groups were higher than that of control group respectively （ P 〈 0.01）. The SUN content of each experimental group was less than that of the control group（ P 〈 0.01, P 〈 0.05）.Moreover,in the medium and high dose experimental groups, less accumulation of lactate was found（ P 〈 0.01, P 〈 0.05）,and the content of liver SOD and GSH-Px was higher （ P 〈 0.01, P 〈 0.05）. The content of liver MDA in high-dose experimental group was less than that of the control group（ P 〈0.05）. Condusion： Shenqi preparation, especially the high and medium-dose experimental groups, is able to improve exercise tolerance and has anti-fatigue and anti-oxidant effects in mice.