Frequency of alleles conferring resistance to Bacillus thuringiensis maize in Louisiana populations of the southwestern corn borer |
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Authors: | Fangneng Huang BRogers Leonard Donald R Cook Donna R Lee David A Andow Jack L Baldwin Kelly V Tindall & Xiaoyi Wu |
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Institution: | Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA,;Northeast Research Station, Louisiana State University Agricultural Center, St. Joseph, LA 71366, USA,;East Carroll Parish Office, Louisiana State University Agricultural Center, Lake Providence, LA 71254, USA,;Department of Entomology, University of Minnesota, St. Paul, MN 55108, USA,;Macon Ridge Research Station, Louisiana State University Agricultural Center, Winnsboro, LA 71295, USA |
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Abstract: | Transgenic maize Zea mays L. (Poaceae)] expressing Bacillus thuringiensis proteins (Bt maize) has become the most important tool for managing stalk borers in maize in the USA. The current strategy for delaying the evolution of resistance in target insects for Bt maize is referred to as high dose/refuge strategy. A key requirement of the strategy is that initial resistance allele frequencies in field insect populations are low (e.g., <0.001). More than 200 iso‐line families of the southwestern corn borer, Diatraea grandiosella Dyar (Lepidoptera: Crambidae), a major target stalk borer pest of Bt maize, were developed from Louisiana populations and evaluated for Bt resistance using a modified F2 screening method during 2005. No major resistance alleles were detected in these populations. The results showed that the expected Bt resistance allele frequency in the Louisiana populations was <0.0035 with 95% probability and a detection power of 83.9 ± 0.6%. The F2 screen indicates that Bt resistance allele frequencies in D. grandiosella are low among the Louisiana populations and should meet the rare resistance allele requirement of the ‘high dose/refuge’ strategy. |
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Keywords: | Diatraea grandiosella resistance detection insecticide resistance management Lepidoptera Crambidae iso-line family Zea mays high dose/refuge strategy |
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