半夏缓慢生长法保存及体细胞变异的ISSR检测

以添加了不同浓度的甘露醇、PP333和ABA的培养基对半夏试管苗进行缓慢生长法保存,并对保存材料再生后代的体细胞变异进行检测。结果显示,甘露醇、PP333和ABA均能有效抑制试管苗生长,且存活率高;最佳浓度分别为甘露醇2%～4%,PP3332.0mg·L-1,ABA 2.0～4.0mg·L-1。保存在添加了2%～4%甘露醇或2.0mg·L-1 PP333培养基上的植株未检测到变异,而保存在添加了2.0～4.0mg·L-1 ABA培养基上的植株检测到1条新增标记和1条缺失标记,位点变异率为1.7%,个体变异率为30%。研究表明,ABA不宜用于半夏试管苗的缓慢生长法保存,但有助于新突变体的产生,在种质创新上具有特殊意义。

Slow Growth Conservation of Pinellia ternata and Its Somatic Variation as Detected by ISSR Markers

Slow growth conservation of Pinellia ternata on medium by adding different concentrations of mannitol,PP₃₃₃,and ABA were carried out.Somatic variation of regenerated plantlets was also studied.The results show that:the three of them all could effectively retard the growth with a high surviving rate.The optimum concentration of mannitol,PP₃₃₃ and ABA were 2%~4%,2.0 mg·L^-1 and 2.0~4.0 mg·L^-1,respectively.No variations were found in the regenerated plantlets which were conserved on medium by adding mannitol 2% or PP₃₃₃ 2.0 mg·L^-1.While,one new marker and one absent marker were found on medium by adding ABA 2.0 mg·L^-1.The locus variation rate was 1.7% and the individual variation rate was 30%.Therefore,ABA was not suggested in slow growth conservation of Pinellia.However,it contributed to the generation of new mutant,and has a special significance in the innovation of P.ternate germplasm.