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An in vitro hepatic zonation model with a continuous oxygen gradient in a microdevice |
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| Authors: | Asako Sato Kanae Kadokura Hideyuki Uchida Kosuke Tsukada |
| Institution: | 1. Graduate School of Fundamental Science and Technology, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama, Kanagawa 223-8522, Japan;2. Department of Applied Physics and Physico-Informatics, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kouhoku-ku, Yokohama, Kanagawa 223-8522, Japan |
| Abstract: | In a hepatic lobule, different sets of metabolic enzymes are expressed in the periportal (PP) and pericentral (PC) regions, forming a functional zonation, and the oxygen gradient is considered a determinant of zone formation. It is desirable to reproduce lobular microenvironment in vitro, but incubation of primary hepatocytes in conventional culture dishes has been limited at fixed oxygen concentrations due to technical difficulties. |
| Keywords: | PP periportal PC pericentral MEMS microelectromechanical systems pO2 partial oxygen pressure GK glucokinase PEPCK phosphoenolpyruvate carboxykinase OCR oxygen consumption rate |
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