Curcumin inhibits oral squamous cell carcinoma SCC-9 cells proliferation by regulating miR-9 expression |
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Authors: | Can Xiao Lili Wang Lifang Zhu Chenping Zhang Jianhua Zhou |
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Institution: | 1. Department of Occupational Medicine and Environmental Health, School of Public Health, Soochow University, Suzhou 215123, China;2. Department of Stomatology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China;3. Department of Head and Neck Tumors, Shanghai Ninth People’s Hospital Affiliated Shanghai JiaoTong University School of Medicine, Shanghai 200011, China |
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Abstract: | Curcumin, a phytochemical derived from the rhizome of Curcuma longa, has shown anticancer effects against a variety of tumors. In the present study, we investigated the effects of curcumin on the miR-9 expression in oral squamous cell carcinoma (OSCC) and explored the potential relationships between miR-9 and Wnt/β-catenin pathway in curcumin-mediated OSCC inhibition in vitro. As the results shown, the expression levels of miR-9 were significantly lower in clinical OSCC specimens than those in the adjacent non-tumor tissues. Furthermore, our results indicated that curcumin inhibited OSCC cells (SCC-9 cells) proliferation through up-regulating miR-9 expression, and suppressing Wnt/β-catenin signaling by increasing the expression levels of the GSK-3β, phosphorylated GSK-3β and β-catenin, and decreasing the cyclin D1 level. Additionally, the up-regulation of miR-9 by curcumin in SCC-9 cells was significantly inhibited by delivering anti-miR-9 but not control oligonucleotides. Downregulation of miR-9 by anti-miR-9 not only attenuated the growth-suppressive effects of curcumin on SCC-9 cells, but also re-activated Wnt/β-catenin signaling that was inhibited by curcumin. Therefore, our findings would provide a new insight into the use of curcumin against OSCC in future. |
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Keywords: | Oral squamous cell carcinoma Curcumin miR-9 Wnt/β-catenin signaling |
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