Entry of Bombyx mori nucleopolyhedrovirus into BmN cells by cholesterol-dependent macropinocytic endocytosis |
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| Authors: | Jinshan Huang Bifang Hao Chen Cheng Fei Liang Xingjia Shen Xiaowen Cheng |
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| Affiliation: | 1. College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, PR China;2. Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang, Jiangsu 212018, PR China;3. Department of Microbiology, Miami University, Oxford, OH 45056, USA |
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| Abstract: | Bombyx mori nucleopolyhedrovirus (BmNPV) is a serious viral pathogen of silkworm, and no drug or specific protection against BmNPV infection is available at present time. Although functions of most BmNPV genes were depicted in recent years, knowledge on the mechanism of BmNPV entry into insect cells is still limited. Here BmNPV cell entry mechanism is investigated by different endocytic inhibitor application and subcellular analysis. Results indicated that BmNPV enters BmN cells by clathrin-independent macropinocytic endocytosis, which is mediated by cholesterol in a dose-dependent manner, and cholesterol replenishment rescued the BmNPV infection partially. |
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| Keywords: | BmNPV, Bombyx mori nucleopolyhedrovirus BV, budded virus ODV, occlusion-derived virus AcMNPV, Autographa californica MNPV CRAC, cholesterol recognition amino acid consensus h p.i, hour post infection MOI, multiplicity of infection DMA, N,N-dimethylacetamide TCID50, 50% tissue culture infective dose MβCD, methyl-β-cyclodextrin RTKs, receptor tyrosine kinases |
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