Effects of nickel oxide on the production of tumor necrosis factor by alveolar macrophages of rats |
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Authors: | Yasuo Morimoto Ziro Nambu Isamu Tanaka Toshiaki Higashi Hiroshi Yamato Hajime Hori Shuji Cho Masamitsu Kido |
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Affiliation: | (1) Department of Environmental Health Engineering, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishiku, 807 Kitakyushi, Japan;(2) Department of Biology, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishiku, 807 Kitakyushu, Japan;(3) Department of Work Systems and Health, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishiku, 807 Kitakyushu, Japan;(4) Division of Respiratory Disease, University of Occupational and Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishiku, 807 Kitakyushu, Japan;(5) Faculty of Home Life Science, Fukuoka Women's University, 1 Kasumigaoka, Higashiku, 813 Fukuoka, Japan |
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Abstract: | To evaluate the effect of green nickel oxide (NiO) on the production of tumor necrosis factor (TNF) by alveolar macrophages, alveolar macrophages were exposed to NiO in vitro and in vivo. For the in vitro study, rats alveolar macrophages were incubated with NiO on a microplate for 24 h. TNF activity in the culture supernatant was determined by the L929 bioassay. Rats alveolar macrophages cultured with 100 and 200 μg/mL of NiO in vitro induced the production of TNF, however, it was not statistically significant compared with the control that was free from NiO exposure. For exposure in vivo, rats were divided into two groups. Five were exposed to a daily concentration of 11.7±2.0 mg/m3 of NiO for an 8-hr/d, 5 d/wk, for 4 wk, and five rats (control) were kept in a cage and not exposed to NiO. Bronchoalveolar lavage was performed and the recovered alveolar macrophages were incubated on a microplate for 24 h. TNF production by exposed alveolar macrophages was significantly higher than that of controls. |
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Keywords: | Nickel oxide tumor necrosis factor inhalation study in vitro assay alveolar macrophage in vitro study |
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