| 一株分离自酸梅酱的酵母菌的分离鉴定 |
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| 引用本文： | 邝海菊,肖婷,何香,兰晓君,艾尔肯·热合曼,索菲娅.一株分离自酸梅酱的酵母菌的分离鉴定[J].生物技术,2010,20(1):48-51. |
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| 作者姓名： | 邝海菊 肖婷 何香 兰晓君 艾尔肯·热合曼 索菲娅 |
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| 作者单位： | 新疆大学生命科学与技术学院,新疆,乌鲁木齐,830046 |
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| 基金项目： | 科技部教学实验用微生物平台项目 |
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| 摘 要： | 目的:分离及鉴定来自于新疆塔城民间自制酸梅酱中的酵母菌。方法:用NL1/NL4引物对扩增酵母菌株的26S rDNAD1/D2区,测序结果进行序列分析,用Neighbour-joining(N-J)方法构建系统发育进化树,同时结合酵母的传统形态学鉴定对菌株进行鉴定。结果:26S rDNA D1/D2区序列分析表明菌株KKS与Metschnikowia aff.fructicola D3895相近,相似率为99.2%。在N-J法构建的系统发育进化树中,菌株KKS与Metschnikowia aff.fructicola聚类在同一分枝上。结论:从新疆塔城民间自制酸梅酱中分离得到一株酵母菌并将该菌株鉴定为Metschnikowia aff.fructicola。
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| 关 键 词： | 酵母 Metschnikowia aff.fructicola 酸梅酱 26S rDNA D1／D2 |
Isolation and Identification a Yeast Strain from Jam of Plum |
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| KUANG Hai-ju,XIAO Ting,HE Xiang,LAN Xiao-jun,Erkin·RAHMAN,SUO Fei-ya.Isolation and Identification a Yeast Strain from Jam of Plum[J].Biotechnology,2010,20(1):48-51. |
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| Authors: | KUANG Hai-ju XIAO Ting HE Xiang LAN Xiao-jun Erkin·RAHMAN SUO Fei-ya |
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| Abstract: | Objective: Yeast from jam of Xinjiang Tacheng's plum was isolated and identified.Method: 26S rDNA D1/D2 domain sequence for yeast strain was amplified by using primer pairs NL1/NL4,the acquired data were used to analyse sequence,and using neighbour-joining method to construct phylogenetic tree.To confirm identification,the morphological and physiological properties were also examined.Result: The sequence analysis of 26S rDNA D1/D2 showed that strain KKS was closely related to Metschnikowia aff.fructicola D3... |
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| Keywords: | Metschnikowia aff fructicola 26S rDNA D1/D2 yeast Metschnikowia aff fructicola jam of plum 26S rDNA D1/D2 |
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