Detection of invasive freshwater fish species using environmental DNA survey |
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| Affiliation: | 1. United States Geological Survey, Upper Midwest Environmental Sciences Center, 2630 Fanta Reed Road, La Crosse WI 54603, USA;2. Whitney Genetics Laboratory, U.S. Fish and Wildlife Service Resource Center, 555 Lester Avenue, Onalaska, WI 54650, USA;3. U.S. Army Corps of Engineers, Engineer Research and Development Center, Environmental Laboratory, 3909 Halls Ferry Road, Vicksburg, MS 39180, USA;4. U.S. Army Corps of Engineers, St. Paul District, Clock Tower Building, P.O. Box 2004, Rock Island, IL 61204, USA;1. U.S. Department of Agriculture, Forest Service, National Genomics Center for Wildlife and Fish Conservation, Rocky Mountain Research Station, Missoula, MT 59801, USA;2. Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA;3. US Geological Survey Northern Rocky Mountain Science Center, Bozeman, MT 59715, USA;4. B.B. Shepard and Associates, Livingston, MT 59047, USA;5. Department of Environmental Conservation, University of Massachusetts, Amherst, MA 01003, USA |
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| Abstract: | Invasive species are one of the most significant problem in freshwater ecosystems. Most common non-native freshwater species in Turkish freshwater fish fauna are Prussian Carp (Carassius gibelio), North African Catfish (Clarias gariepinus), Nile Tilapia (Oreochromis niloticus) and Topmouth Gudgeon (Pseudorasbora parva).Recent studies showed that environmental DNA could be used to detect target species inhabiting the ecosystem with higher precision and less effort compared to traditional field surveys. In this study, eDNA approach was used to investigate non-native freshwater fish species from fifteen different locations of Upper Sakarya Basin. eDNA was successfully extracted from the water samples of locations where the species were visually observed. Mean amplification rate of eDNA was calculated as 77.03%.This study is the first environmental DNA study used in detection of four of the most common invasive freshwater fish species. Results clearly indicating that eDNA surveys could be used as an important molecular tool to monitor invasive fish species in freshwater ecosystems. |
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| Keywords: | eDNA Invasive species |
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