Characterization of 11 novel polymorphic microsatellite loci in the threatened Korean loach,Iksookimia koreensis,isolated using a next-generation sequencing method |
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| Affiliation: | 1. National Institute of Biological Resources, Environmental Research Complex, Gyeongseo-dong, Seo-gu, Incheon 404-708, South Korea;2. Department of Biological Science, College of Natural Science, Chonbuk National University, Jeonju 561-756, South Korea;1. Ioffe Institute, ul. Polytechnitscheskaya 26, St.-Petersburg, 194021, Russia;2. Tambov State Technical University, ul. Sovetskaya 106, Tambov, 392000, Russia;1. Department of Pain Management, The Affiliated Hospital of Qingdao University, Qingdao 266003, China;2. Department of Anesthesiology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China;3. Chinese Traditional Patent Medicine Dispensary, The Affiliated Hospital of Qingdao University, Qingdao 266003, China;1. Laboratorio de Química Física y Catálisis Computacional, Centro de Química, Instituto Venezolano de Investigaciones Científicas, Apartado, 21827 Caracas, Venezuela;2. Laboratorio de Físico Química Teórica de Materiales, Centro de Química, Instituto Venezolano de Investigaciones Científicas, Apartado, 21827 Caracas, Venezuela;3. Instituto Universitario de Tecnología de Valencia IUTVAL, Valencia, Edo. Carabobo, Venezuela;1. Institute for Nuclear Waste Disposal (KIT-INE), Karlsruhe Institute of Technology, P.O. Box 3640, 76021 Karlsruhe, Germany;2. Institute for Nuclear and Energy Technologies (KIT-IKET), Karlsruhe Institute of Technology, P.O. Box 3640, 76021 Karlsruhe, Germany |
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| Abstract: | To investigate the genetic diversity and population structure of Iksookimia koreensis, we characterized 11 novel polymorphic microsatellite markers developed using next-generation sequencing. The number of alleles per locus ranged from 4 to 10 (average = 6.26). Observed and expected heterozygosities ranged from 0.333 to 0.866 and from 0.375 to 0.866, respectively. No loci showed significant deviation from Hardy–Weinberg equilibrium (HWE). These loci were also used successfully to study the genetic diversity of a closely related species, Iksookimia longicorpa. Four of the 11 loci amplified in the two species showed different allele frequency and distribution, indicating deep genetic divergence between I. koreensis and I. longicorpa. The newly developed microsatellite markers reported here will provide a useful tool for examining gene flow, population genetic structure, and genetic diversity of these species. |
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| Keywords: | Next-generation sequencing Microsatellites Cross-species amplification |
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