Genetic diversity of the threatened Chinese endemic plant,Sinowilsonia henryi Hemsi. (Hamamelidaceae), revealed by inter-simple sequence repeat (ISSR) markers |
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| Affiliation: | 1. College of Life Sciences and Engineering, Shaanxi University of Technology, Hanzhong 723001, PR China;2. Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life Sciences, Northwest University, Xi''an 710069, PR China;3. College of Life Sciences, Shaanxi Normal University, Xi''an 710119, PR China;4. College of Life Sciences and Technology, Tongji University, Shanghai 200092, PR China;1. Department of Endocrinology, Diabetes and Metabolic Diseases “Mladen Sekso”, University Hospital Center “Sestre Milosrdnice”, Zagreb, Croatia;2. University of Zagreb School of medicine, Zagreb, Croatia;1. INRIM, Electromagnetics Division, Strada delle Cacce 91, 10135 Torino (TO), Italy;2. Università degli Studi di Torino, Dipartimento di Chimica, via P. Giuria 7, 10125 Torino (TO), Italy;1. Department of Physics, GTN Arts College, Dindigul-624 005, India;2. Post Graduate and Research Department of Physics, Government Arts College, Melur-625106, Madurai, India;3. Department of Chemical Engineering, College of Engineering, Kyung Hee University, 1732 Deogyeong-daero, Gihung, Yongin, Gyeonggi, 446-701, South Korea;1. International Joint Laboratory on Tea Chemistry and Health Effects, State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, PR China;2. Cancer Prevention Laboratory, Colorado State University, Fort Collins, CO, USA |
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| Abstract: | Sinowilsonia henryi Hemsi., the only representative of the monotypic genus Sinowilsonia Hemsi. (Hamamelidaceae), is a threatened plant endemic to China with high phylogenetical, ecological and economical values. In the present study, inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 214 individuals sampled from 14 populations. Fifteen selected primers yielded a total of 178 bright and discernible bands. The genetic diversity was low at the population level (h = 0.1025; I = 0.1506; PPL = 26.7%), but quite high at the species level (h = 0.2449; I = 0.3690; PPL = 72.5%). In line with the limited gene flow (Nm = 0.3537), the hierarchical analysis of molecular variance (AMOVA) revealed pronounced genetic differentiation among populations (ΦST = 0.6639). Furthermore, the Mantel test revealed a significant correlation between genetic and geographic distances among populations (r = 0.688, P = 0.001), indicating the role of geographic isolation in shaping its present population genetic structure. The present patterns of genetic diversity of S. henryi were assumed to result largely from its evolutionary history and geographic factors. Based on these findings, conservation strategies were proposed to preserve this threatened plant. |
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| Keywords: | Genetic diversity Genetic differentiation Endemic species Inter-simple sequence repeats (ISSRs) |
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