Development and application of 15 novel polymorphic microsatellite markers for sect. Paeonia (Paeonia L.) |
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| Affiliation: | 1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, No. 35 Qinghuadong Road, Beijing 100083, PR China;2. Miki-cho Post Office, P. O. Box 7, Ikenobe 3011-2, Kagawa-ken 761-0799, Japan;1. Departamento de Farmacología, Facultad de Medicina, C. Escolar, C. Universitaria, Coyoacán, 04510 Mexico, D. F, Mexico;2. Instituto de Química, C. Exterior, C. Universitaria, Coyoacán, 04510 Mexico, D. F, Mexico;1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China;2. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;1. Department of Biology, Gyeongsang National University, Jinju 660-701, Republic of Korea;2. Department of Biology and the Research Institute of Natural Science, Gyeongsang National University, Jinju 660-701, Republic of Korea;1. Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China;2. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China |
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| Abstract: | Herbaceous peony, which belongs to sect. Paeonia, Paeonia L., Paeoniaceae, is not only a famous traditional flower in China; it is also highly regarded as an ornamental in Europe and the USA. On account of the abundance in germplasm resources and wide distribution ranges, herbaceous peony can be divided into three cultivar groups, the Chinese Peony Cultivar Group, the European Peony Cultivar Group and the Hybrid Peony Cultivar Group. However, most studies on genetic relationships in Paeonia are limited to the first group. This study used 15 polymorphic microsatellite markers that were developed by magnetic bead enrichment to explore the genetic diversity of 89 genotypes collected from Beijing, the USA and Canada and introduced to China. From 145 allelic loci that were detected from 15 primers, 140 (96.6%) were polymorphic. The number of allelic loci ranged from 4 to 16 with an average of 9.3, and the polymorphic index content ranged from 0.362 to 0.825 with an average of 0.678. Both cluster analysis and principal component analysis based on the 15 polymorphic primer pairs could distinguish the species from cultivars although the cluster analysis was better able to reflect the genetic relationships between all individuals. Some new insights about Paeonia L. are suggested. Furthermore, the 15 SSR markers have great significance as they will allow for furthering the studies on genetic diversity, genetic relationships and even the molecular marker-assisted breeding of Paeonia L. |
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| Keywords: | 15 polymorphic SSR markers Genetic diversity of 89 genotypes Herbaceous peony Magnetic bead enrichment AFLP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0035" }," $$" :[{" #name" :" text" ," _" :" amplified fragment length polymorphism Shannon's diversity index ISSR" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" inter-simple sequence repeat number of alleles effective number of alleles polymorphism ratio PCA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" principal component analysis PIC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0105" }," $$" :[{" #name" :" text" ," _" :" polymorphism index content RAPD" },{" #name" :" keyword" ," $" :{" id" :" kwrd0115" }," $$" :[{" #name" :" text" ," _" :" random amplified polymorphic DNA SSR" },{" #name" :" keyword" ," $" :{" id" :" kwrd0125" }," $$" :[{" #name" :" text" ," _" :" simple sequence repeat |
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