Genetic diversity assessment and estimation of phylogenetic relationships among 26 Fagaceae species using ISSRs |
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| Affiliation: | 1. Department of Animal Production, School of Agriculture, Faculty of Agriculture, Forestry & Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, Greece;2. Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece;3. Department of Zoology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece;1. Departamento de Farmacología, Facultad de Medicina, C. Escolar, C. Universitaria, Coyoacán, 04510 Mexico, D. F, Mexico;2. Instituto de Química, C. Exterior, C. Universitaria, Coyoacán, 04510 Mexico, D. F, Mexico;1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China;2. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;1. Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Huachiew Chalermprakiet University, Samutprakarn 10540, Thailand;2. Nationaal Herbarium Nederland, Leiden University, The Netherlands;3. Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand;4. Département de Physico-chimie du Medicament, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, Paris 75006, France;5. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand |
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| Abstract: | The family Fagaceae includes several species and presents huge genetic variability. In the last two decades, several genetic studies about phylogenetics and genetic diversity of Fagaceae have emerged. ISSR markers were used to evaluate the genetic diversity of 26 species of Fagaceae belonging to the genera Castanea, Fagus and Quercus. Among several primers tested, 17 were selected for the evaluation of diversity and estimation of genetic relationships. A total of 371 ISSR markers were produced and each primer revealed high polymorphism. Specific ISSR markers for the Quercus infrageneric groups were amplified. ISSRs proved to be a reliable tool for the discrimination of the analyzed species per genus, infrageneric group and/or ecological origin. |
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| Keywords: | Genetic variability Inter-simple sequence repeats (ISSRs) |
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