Application of DNA barcoding in Roscoea (Zingiberaceae) and a primary discussion on taxonomic status of Roscoea cautleoides var. pubescens |
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| Affiliation: | 1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, PR China;2. College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404000, PR China;1. Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China;2. Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang Province 311121, China;1. Institute of Chemistry, São Paulo State University, UNESP, 14801-970, Araraquara, SP, Brazil;2. Embrapa Cattle – Southeast, CP 339, 13560-970, São Carlos, SP, Brazil;1. AZTI, Marine Research Division, Sukarrieta, Bizkaia, Spain;2. King Abdullah University of Science and Technology, Red Sea Research Center, Biological and Environmental Sciences & Engineering Division, Thuwal, Saudi Arabia;1. Department of Botany, Faculty of Science, Charles University, Prague, Czech Republic;2. Department of Botany, National Museum in Prague, Prague, Czech Republic;3. Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovak Republic;4. The Herbarium, Singapore Botanic Gardens, Singapore |
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| Abstract: | DNA barcoding is a useful tool to define operational taxonomic units based on standardized DNA regions. In this study, three chloroplast markers (rbcL, trnH-psbA and matK) and one nrDNA marker (ITS) were tested for species identification in Roscoea. The ITS and trnH-psbA regions showed high success rate of PCR amplification and bidirectional sequencing, as well as perfect discriminatory ability. On the contrary, rbcL possessed no genetic variation and matK was relatively difficult in PCR amplification and DNA sequencing. Combination of multiple markers greatly improved identification ability of DNA barcoding. ITS + trnH-psbA could effectively discriminate 90% species based on method of the Neighbor-Joining (NJ) tree. Awful PCR amplification and DNA sequencing of matK restricted its efficiency, although it showed rich genetic variability in Roscoea. Moreover, it might be more appropriate to treat Roscoea cautleoides var. pubescens as an independent species based on molecular data, namely R. pubescens Z. Y. Zhu. |
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| Keywords: | DNA barcoding Species identification Neighbor-Joining (NJ) tree |
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