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Morphometric,meristic and ISSR marker systems for species identification and evolutionary analysis in five Indian Channids |
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| Institution: | 1. Research and Collections, New York State Museum, 222 Madison Ave, Albany, NY 12230, USA;2. Department of Anthropology, University at Albany – SUNY, 1400 Washington Ave, Albany, NY 12222, USA;3. Department of Archaeology and Palaeoanthropology, University of New England, Armidale, NSW 2351, Australia;1. Departamento de Geología, Universidad de Oviedo, C/Jesús Arias de Velasco, s/n, 33005, Oviedo, Spain;2. Departamento de Biología Funcional, Universidad de Oviedo, C/Julián Clavería s/n, 33006, Oviedo, Spain |
| Abstract: | Snakehead species belonging to Channidae are primary group of freshwater air breathing fishes having their confined distribution in African and Asian continents. ISSR – PCR was used to investigate the phylogenetic relationship among five Channidae species viz. Channa striatus, Channa marulius, Channa punctatus, Channa diplogramme and Channa gachua. In addition, morphometric and meristic characters were subjected to principal component analysis (PCA) and the bootstrap values within the species were also calculated. The genetic identity between the species ranged from 0.5526 to 0.7632 and the genetic distance ranged from 0.2703 to 0.5931. The Nei's gene diversity (H) was calculated as 0.2653 and the Shannon's information index (I) was 0.3842. UPGMA dendrogram arrived by the morphological and molecular markers revealed the closeness between C. striatus and C. marulius among the five species. |
| Keywords: | Murrels ISSR marker Genetic variation Evolutionary analysis DNA"} {"#name":"keyword" "$":{"id":"kwrd0035"} "$$":[{"#name":"text" "_":"Deoxyribo Nucleic Acid ISSR"} {"#name":"keyword" "$":{"id":"kwrd0045"} "$$":[{"#name":"text" "_":"Inter-Simple Sequence Repeats PCA"} {"#name":"keyword" "$":{"id":"kwrd0055"} "$$":[{"#name":"text" "_":"Principal Components Analysis PCR"} {"#name":"keyword" "$":{"id":"kwrd0065"} "$$":[{"#name":"text" "_":"Polymerase Chain Reaction PAST"} {"#name":"keyword" "$":{"id":"kwrd0075"} "$$":[{"#name":"text" "_":"Paleontological Statistics Software RAPD"} {"#name":"keyword" "$":{"id":"kwrd0085"} "$$":[{"#name":"text" "_":"Randomly Amplified Polymorphic DNA UPGMA"} {"#name":"keyword" "$":{"id":"kwrd0095"} "$$":[{"#name":"text" "_":"Unweighed Pair Group Method with Arithmetic Average |
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