Isozyme variation and differentiation of morphologically cryptic species in the Brachypodium distachyon complex |
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| Institution: | 1. Laboratório de Genômica Evolutiva e Ambiental, Universidade Federal de Pernambuco, Centro de Ciências Biológicas, Departamento de Zoologia, Recife, PE, Brazil;2. Universidade Federal do Pará, Laboratório de Genética e Biologia Molecular, Instituto de Estudos Costeiros, Bragança, Pará, Brazil;3. Departamento de Geociências, Centro de Filosofia e Ciências Humanas, Universidade Federal de Santa Catarina, Florianópolis, SP, Brazil;4. Universidade Federal de Pernambuco, Centro de Ciências Biológicas, Departamento de Zoologia, Recife, PE, Brazil;5. Universidade Federal do Paraná, Programa de Pós-graduação em Ecologia e Conservação, Niterói, RJ, Brazil;6. Universidade Federal de Pernambuco, Departamento de Oceanografia, Niterói, RJ, Brazil;7. Universidade Federal do Espírito Santo, CEUNES, Departamento de Ciências Agrárias e Biológicas, Niterói, RJ, Brazil;8. Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Programa de Pós-Graduação Biodiversidade Neotropical, Av. Pasteur, 458, CCET/IBIO, Urca, Rio de Janeiro/RJ 22290-240, Brazil;9. Institut Français de Recherche Pour I''exploitation de la Mer, IFREMER, France;1. Institute of Health Economics, Edmonton, Alberta, Canada;2. Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada;3. Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada;1. Department of Phytochemistry and Plant Systematics, National Research Centre, Dokki, Giza 12311, Egypt;2. Department of Botany, Faculty of Sciences, Cairo University, Giza 12613, Egypt;3. Department of Biology, Faculty of Sciences, Taibah University, Al Madinah Al Munawwarah, P.O. Box 344, Saudi Arabia;1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, Telangana, India;2. National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India;1. Wellcome Trust Centre for Cell Biology, The University of Edinburgh, Edinburgh EH9 3JR, UK;2. The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh EH25 9RG, UK;3. Centre for Synthetic and Systems Biology (SynthSys), University of Edinburgh, Edinburgh EH9 3JD, UK |
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| Abstract: | Isozyme variation of nine enzymes among three morphologically cryptic species of the Brachypodium distachyon complex was studied with PAGE. Aspartate aminotransferase isozymes AAT-A and AAT-C, aryl alcohol dehydrogenase AAD-B, malate dehydrogenase MDH-A and leucine aminopeptidase LAP-A in the allotetraploid B. hybridum display fixed heterozygosities with codominant expression of homoeozymes corresponding to divergent orthozymes of diploids B. distachyon and B. stacei. The multilocus isozyme genotype inherent to most accessions of B. hybridum combines two divergent multilocus isozyme genotypes characteristic of most accessions of B. distachyon and B. stacei. The maternally inherited chloroplast-encoded RUBISCO isozymes show that B. stacei is a maternal progenitor of B. hybridum, whereas the nuclear-encoded isozymes AAD-B, AAT-A, AAT-C, LAP-A, and MDH-A confirm that B. distachyon is the paternal progenitor. These data provide a new isozyme-based support to the karyological and DNA marker data on the allotetraploid nature of B. hybridum and its origin from diploids B. distachyon and B. stacei. |
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| Keywords: | Cryptic species Isozymes Allopolyploidy |
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