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Limited genetic differentiation in Labeo rohita (Hamilton 1822) populations as revealed by microsatellite markers
Affiliation:1. Fish Genetics and Biotechnology Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751002, Odisha, India;2. University of Chile, Santiago, Chile;1. Wihuri Physical Laboratory, Department of Physics and Astronomy, University of Turku, FI-20014 Turku, Finland;2. Department of Applied Physics, Aalto University School of Science, P.O. Box 15100, FI-00076 Aalto, Finland;1. National Engineering Laboratory for Next Generation Internet Access System (HUST), Wuhan 430074, PR China;2. School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan 430074, PR China
Abstract:Labeo rohita, popularly known as rohu is a widely cultured species in the whole Indian subcontinent. Knowledge of the genetic diversity of this species is important to support management and conservation programs which will subsequently help in sustainable production of this species. DNA markers, mostly microsatellite markers are excellent tool to evaluate genetic variation of populations. Genetic variation of three wild and one farm population was assessed using eleven microsatellite loci. In analyzing 192 samples, the number of alleles ranged from 4 to 23; observed heterozygosity 0.500 to 0.870 and expected heterozygosity from 0.389 to 0.878. Exact test for Hardy Weinberg disequilibrium revealed that each riverine sample had at least one locus not in equilibrium except one river. Negative inbreeding coefficients (FIS) were observed across populations indicating very high level of genetic diversity but little genetic differentiation among populations.
Keywords:Indian major carp  Genetic diversity  Microsatellite  Reverine population
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