Recombination between kappa chain genetic markers in the mouse |
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| Authors: | D M Gibson S J MacLean D Anctil B J Mathieson |
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| Institution: | (1) Département de Biochimie, Université de Sherbrooke, Sherbrooke, Québec, Canada;(2) The Laboratory of Microbial Immunity, National Institute of Allergy and Infectious Diseases, 20205 Bethesda, Maryland;(3) Present address: National Cancer Institute, Biological Response Modifiers Program, Frederick Cancer Research Facility, Building 560, Room 31-93, 20217 Frederick, MD |
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| Abstract: | In this study we report the first instance of recombination between kappa chain genetic markers in the mouse. The recombination frequency, 0.45% (95% limits, 0.12–1.61), is similar to that previously found for recombination between the kappa chain locus and the Lyt-2, 3 locus (0.3%, 95% limits, 0.05–1.6), but is relatively low in comparison with that found at the heavy chain locus (0.41–5.4%). Lyt-2, 3-typing of the recombinants permits a partial ordering of the kappa chain and Lyt-2, 3 loci as (Lyt-2, 3, Igk-Ef1) - Igk-Ef2. Light chains controlled by the two kappa markers include the Vk-(ser) subgroup (controlled by Igk-Ef1) and Vk–1 (controlled by Igk-Ef2). One of the recombinants has been recovered in a homozygous state ( NAK ) and should be suitable for V
k
gene mapping studies.Abbreviations C
complement
- CH
constant region of the Ig heavy chain
- CI
cytotoxicity index
- DNP
dinitrophenyl
- FMF
flow microfluorimetry
- IEF
isoelectric focusing
- IF
immunofluorescence
- Ig
immunoglobulin
- KLH
keyhole limpet hemocyanin
- VH
variable region of the Ig heavy chain
- Vk
variable region of the Ig kappa chain
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V-region
variable region |
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